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1.
Journal of Veterinary Science ; : 47-57, 2002.
Article in English | WPRIM | ID: wpr-16605

ABSTRACT

Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems remained, especially in terms of reduced laying rates. Therefore, experimental vaccines were reformulated with various bacterial antigens of the swine respiratory diseases. After immunizing hens with the antigens, antibody profiles and other effects including laying rates were investigated and compared to those of the previous study. Profiles of antibody titers were very similar with those of the previous study. However, side effects, such as depression, weakness, reduction of laying rates and mortality, were dramatically lowered and laying rates were increased in hens injected with certain experimental vaccines. In particular, laying rates of hens injected with vaccines against atrophic rhinitis were increased up to 84% by injecting a vaccine composed of only the DNTs of B. bronchiseptica and P. multocida D:4. Efficacies of the vaccines against swine pneumonic pasteurellosis and pleuropneumonia were very similar with those of the previous study. These results suggest that new vaccines could be effective in the production of egg yolk antibodies against the causative agents of swine respiratory diseases.


Subject(s)
Animals , Female , Actinobacillus pleuropneumoniae/classification , Antibodies, Bacterial , Antibody Formation , Bacterial Outer Membrane Proteins/genetics , Bordetella bronchiseptica/classification , Egg Yolk/microbiology , Immunoglobulins/genetics , Oviposition , Pasteurella multocida/classification , Serotyping , Swine
2.
Rev. microbiol ; 27(3): 155-60, set. 1996.
Article in English | LILACS | ID: lil-193548

ABSTRACT

Estudou-se a dinâmica de crescimento e síntese de polissacarídios capsulares de uma amostra de Actinobacillus pleuropneumoniae sorotipo 5 em diferentes condiçöes de cultivo. A bactéria foi multiplicada em caldo infuso de cérebro e coraçäo (BHI) e caldo de soja e tripticaseina (TSB), suplementados com extrato de levedura. Realizaram-se cultivos em frascos Erlenmeyer estáticos em atmosfera de CO2 e agitados em aerobiose, e em fermentador com e sem aeraçäo. Determinou-se a curva de crescimento, concentraçäo de hexosaminas, aderência a n-Hexadecano e a demanda de oxigênio em diferentes períodos de incubaçäo. A fase logarítmica de crescimento terminou mais rapidamente, e a produçäo de biomassa e de hexosaminas foram maiores nos cultivos realizados em condiçöes de aerobiose. A maior demanda de oxigênio coincidiu com o término da fase exponencial de crescimento. A aderência ao n-Hexadecano näo teve relaçäo com a fase de cultivo nem com a concentraçäo de polissacarídios capsulares


Subject(s)
Polysaccharides, Bacterial , Serotyping , Actinobacillus pleuropneumoniae/classification , Bacterial Adhesion/immunology
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